E.coli Protein Expression Service
E.coli Protein Expression Service
E.coli protein expression system is now the most wide-used and economical expression system. E.coli cell possesses features of clear genetic background, high expression of target genes, short cycle, strong anti-pollution ability and easy to be scaled up. E.coli protein expression system is suitable for protein expression of multiple species, acting as a principal tool for recombinant protein expression and biotechnological industrial development, especially for small protein production. With abundant experience of soluble protein expression and production and protein refolding, and multiple E.coli cell lines and expression vectors, Sino Biological can provide you high-quality expression service.
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E.coli Protein Expression Service Details
| Service procedures | Specification | Timeline | Deliverables | Pricing |
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| ① Gene synthesis and codon optimization (optional) |
1-2 weeks | |||
| ② Vector construction |
• clone cDNA into expression vector • plasmid sequencing • large-scale plasmid preparation |
1-2 weeks | • Sequencing report | |
| ③ Expression evaluation |
• transformation into proper strain • small-batch fermentation • SDS-PAGE analysis to evaluate protein expression |
1-2 weeks | • Expression evaluation result | |
| ④ Protein expression and purification |
• fermentation based on expression evaluation • protein purification soluble expression-affinity purification inclusion body expression-refolding • SDS-PAGE and UV analysis |
2-3 weeks | • Feasibility report • If expression is feasible, provide a certain amount of protein sample to the client based on the expression. |
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| ⑤ Large-scale expression and purification (optional) |
2-3 weeks | • Purified protein • SDS-PAGE report • CoA |
Case Studies of E.coli Protein Expression Service
Case study of E.coli Protein A Expression
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Reduced SDS-PAGE >95% 
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SEC-HPLC >98.5% 
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Cell bioactivity: EC50=17pg/mL 
E.coli Protein Expression Service Advantages
- Years of experience of protein expression
- Multiple protein purification and refolding technologies
- High-throughput and large-scale protein expression and production
- One-stop service from gene to purified protein
- Multiple expression vectors and E.coli cells to meet various expression needs
E.coli Protein Expression Service Procedures
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Communicate about protein expression requirements |
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Sign contract and make prepayment |
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Carry out pilot study for protein expression |
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Feedback pilot study result and ship sample (if feasible) |
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Bulk production quotation |
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Bulk production based on customer requirements |
Contact Us
Tel
215-583-7898
Fax
+86-010-50953280
Email
cro_us@sinobiological.com
