E.coli Protein Expression Service

E.coli Protein Expression Service

E.coli protein expression system is now the most wide-used and economical expression system. E.coli cell possesses features of clear genetic background, high expression of target genes, short cycle, strong anti-pollution ability and easy to be scaled up. E.coli protein expression system is suitable for protein expression of multiple species, acting as a principal tool for recombinant protein expression and biotechnological industrial development, especially for small protein production. With abundant experience of soluble protein expression and production and protein refolding, and multiple E.coli cell lines and expression vectors, Sino Biological can provide you high-quality expression service.

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E.coli Protein Expression Service Details

Service procedures Specification Timeline Deliverables Pricing

Gene synthesis and codon optimization (optional)
  1-2 weeks  

Vector construction
• clone cDNA into expression vector
• plasmid sequencing
• large-scale plasmid preparation
1-2 weeks • Sequencing report

Expression evaluation
• transformation into proper strain
• small-batch fermentation
• SDS-PAGE analysis to evaluate protein expression
1-2 weeks • Expression evaluation result

Protein expression and purification
• fermentation based on expression evaluation
• protein purification
  soluble expression-affinity purification
  inclusion body expression-refolding
• SDS-PAGE and UV analysis
2-3 weeks • Feasibility report
• If expression is feasible, provide a certain amount of protein sample to the client based on the expression.

Large-scale expression and purification (optional)
  2-3 weeks • Purified protein
• SDS-PAGE report
• CoA

Case Studies of E.coli Protein Expression Service

Case study of E.coli Protein A Expression

  • Reduced SDS-PAGE >95%
  • SEC-HPLC >98.5%
  • Cell bioactivity: EC50=17pg/mL

E.coli Protein Expression Service Advantages

  • Over 10 years' experience of protein expression
  • Multiple protein purification and refolding technologies
  • High-throughput and large-scale protein expression and production
  • One-stop service from gene to purified protein
  • Multiple expression vectors and E.coli cells to meet various expression needs

E.coli Protein Expression Service Procedures

Communicate about protein expression requirements
Sign contract and make prepayment
Carry out pilot study for protein expression
Feedback pilot study result and ship sample (if feasible)
Bulk production quotation
Bulk production based on customer requirements

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