Custom Phospho-Specific Antibody Production Service
Phospho-Specific Antibody Introduction
Post-translational modification, especially phosphorylation of proteins critical for the biological function of proteins . It plays an important role in many key regulatory mechanisms of life phenomena such as signaling, cell cycle, proliferation, metabolism, growth & development and cancer mechanisms.
Phosphorylation process is usually the addition of a phosphate (PO4) group to a protein, commonly at a serine (S), threonine (T) or tyrosine (Y) residue via the action of a kinase. Phosphorylation-Specific Antibody can discriminate between the phosphorylated and the non-phosphorylated state of a protein. It is one of the most frequently asked for types of PTM specific antibodies.
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Phospho-Specific Antibody Production Service Items & Procedures
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Design and synthesis of phosphopeptide |
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Animal immunization |
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Monoclonal antibody screening & library construction |
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Antibody production and purification |
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Quality verification & delivery |
Three Technical Platforms for Phospho-Specific Antibody Production Service
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Phospho-Specific Rabbit pAb Production Platform
Short cycle, low price
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Phospho-Specific Mouse mAb Production Platform
High specificity and good lot-to-lot consistency
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Phospho-Specific Rabbit mAb Production Platform
High affinity and sensitivity
Delivery Standards of Phospho-Specific Rabbit pAb Production Service
| Service Name | Final Deliverables | Guarantee | Pricing |
|---|---|---|---|
| Phospho-Specific Rabbit pAb Production Service | • Antiserum or 1~3 mg phospho-specific antibody • 2~4 mg phosphopeptide（>90% purity) • 2~4 mg non-phosphopeptide（>90% purity) • Experimental report |
• ELISA titer≥1:64,000 • Cross-reactivity with non-phosphopeptide <10% |
Applications of Phospho-Specific Antibody
Protein phosphorylation levels are often associated with protein activity, many signaling pathway proteins cause a series of cellular responses by altering their phosphorylation levels after some stimulation, but their protein content does not change much.
Phospho-specific antibody, Prepared against a specific phosphorylation site to detect the changes in the functional state of the protein (enhanced or decreased phosphorylation level) when the cell is stimulated.
Non-phospho-specific antibody, it mainly detects the protein expression level, tissue distribution or cell localization in tissues or cells, and can also be used as an internal reference for changes in phosphorylation level.
