Custom Phospho-Specific Antibody Production Service
Phospho-Specific Antibody Introduction
Post-translational modification, especially phosphorylation of proteins critical for the biological function of proteins . It plays an important role in many key regulatory mechanisms of life phenomena such as signaling, cell cycle, proliferation, metabolism, growth & development and cancer mechanisms.
Phosphorylation process is usually the addition of a phosphate (PO4) group to a protein, commonly at a serine (S), threonine (T) or tyrosine (Y) residue via the action of a kinase. Phosphorylation-Specific Antibody can discriminate between the phosphorylated and the non-phosphorylated state of a protein. It is one of the most frequently asked for types of PTM specific antibodies.
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Phospho-Specific Antibody Production Service Items & Procedures
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| Design and synthesis of phosphopeptide | Animal immunization | Monoclonal antibody screening & library construction | Antibody production and purification | Quality verification & delivery |
Three Technical Platforms for Phospho-Specific Antibody Production Service
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Phospho-Specific Rabbit pAb Production Platform
Short cycle, low price
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Phospho-Specific Mouse mAb Production Platform
High specificity and good lot-to-lot consistency
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Phospho-Specific Rabbit mAb Production Platform
High affinity and sensitivity
Delivery Standards of Phospho-Specific Rabbit pAb Production Service
| Service Name | Final Deliverables | Guarantee | Pricing |
|---|---|---|---|
| Phospho-Specific Rabbit pAb Production Service | • Antiserum or 1~3 mg phospho-specific antibody • 2~4 mg phosphopeptide（>90% purity) • 2~4 mg non-phosphopeptide（>90% purity) • Experimental report |
• ELISA titer≥1:64,000 • Cross-reactivity with non-phosphopeptide <10% |
Applications of Phospho-Specific Antibody
- Protein phosphorylation levels are often associated with protein activity: Many signaling pathway proteins cause a series of cellular responses by altering their phosphorylation levels after some stimulation, but their protein content does not change much.
- Phospho-specific antibody: Prepared against a specific phosphorylation site to detect the changes in the functional state of the protein (enhanced or decreased phosphorylation level) when the cell is stimulated.
- Non-phospho-specific antibody: It mainly detects the protein expression level, tissue distribution or cell localization in tissues or cells, and can also be used as an internal reference for changes in phosphorylation level.
