• Experienced in the successful development and production of about 6000 recombinant proteins.
• One-stop service from gene to protein
• Multiple protein expression systems available including bacteria, yeast, baculovirus-insect and mammalian expression systems
• Multiple purification systems (30+) from which to choose
• Comprehensive research and development capabilities with over 1000 new proteins generated per year
• 80+ bioreactors with various volume capacities ranging from 2 to 1000 L to meet high-throughput and large-scale production requirements
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Overview of Recombinant Protein Expression: Key Influencing Factors, Case Studies and FAQs |
Download Sino Biological's newest Brochure (2019): Strategies to Obtain High-Quality Recombinant Proteins: Protocols and Case Studies |
Case study of E.coli protein A expression More details>>>
SEC-HPLC >98.5% |
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Cell bioactivity: EC50=17pg/mL |
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Case study of Baculovirus-Insect protein A expression More details>>>
SEC-HPLC >93% |
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Cell bioactivity: EC50=7ng/mL |
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Case study of HEK293 transient protein A expression More details>>>
SEC-HPLC >98.2% |
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SDS-PAGE >98.7% |
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Transient protein expression service is great! The purity is as good as I requested. |
Am***a Wa***n December 20, 2020 |
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Consistent and reliable protein expression services with rapid turnaround time. |
El*** De***gina October 14, 2020 |
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The protein service group is awesome! Thank you for efficient work on my protein expression. |
Ed***d Nu*** July 31, 2020 |
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Sino Biological is a wonderful company! I'll continue to work with. |
Eu***e Ki* December 6, 2019 |
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Nice communication. High quality results delivered in a timely manner. |
Za** Ma***man July 13, 2019 |
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The team at Sino Biological is very knowledgeable and professional. They are quick to adapt to any requests and issues. |
Ro***ta Cl*** January 27, 2019 |
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Sino Biological is quick to respond and patient to my questions. The reports are very clear and thorough. |
Me***n Ho***s August 10, 2018 |
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Great job! I'll use this service again to get more proteins. |
Ro***d Me***z May 26, 2018 |
E.coli Protein Expression Service |
• Years of experience of protein expression |
• Multiple protein purification and refolding technologies | |
• High-throughput and large-scale protein expression and production | |
• One-stop service from gene to purified protein | |
• Multiple expression vectors and E.coli cells to meet various expression needs |
Mammalian Transient Protein Expression Service |
• High-efficiency expression vectors |
• Optimized mammalian expression systems | |
• High-throughput and large-scale production | |
• High yield expression with post-translational modification and correct folding | |
• One-stop service from gene sequence to purified protein |
Baculovirus-Insect Cell Protein Expression Service |
• High-efficiency expression of intracellular and extracellular proteins |
• Multiple insect cells: Sf9,Sf21,Hi-5 | |
• High-throughput and large-scale protein production | |
• Optimized expression vectors and higher-titre virus packaging |
Bacterial, yeast, insect and mammalian protein expression systems are widely used. Each system has its advantages and challenges. For successful recombinant protein expression, it is important to choose the right system for a specific application. Protein type, function and desired protein yield will also influence the choice of the system.
Expression Systems | E.coli | Yeast | Baculovirus-insect | Mammalian |
Prokaryotic | Eukaryotic | Eukaryotic | Eukaryotic | |
Advantages | • Low cost • Rapid expression • Easy scale-up • Most widely used system for recombinant protein production |
• Low cost • Rapid expression • High yield • Diverse post-translational modifications |
• High capacity genes • Soluble proteins • Suitable for toxic proteins • Post-translational modifications similar to mammalian systems |
• Soluble protein • Lower endotoxin • Better bioactivity • Comprehensive post-translational modifications • Transient & stable expression |
Challenges | • Inclusion bodies • Lack of eukaryotic post-translational modifications • Difficult to express higher MW proteins |
• Improper glycosylation • High mannose modification |
• More demanding culture conditions • Lack of partial glycosylation |
• More demanding culture conditions |
Most Common Applications | • Bacterial proteins • Antigen proteins • Cytokines • Enzymes |
• Cytokines • Lower MW proteins • Enzymes |
• Cytoplasmic proteins • Toxic proteins • Transmembrane proteins • Secretory proteins • Kinases |
• Secretory proteins • Extracellular domain of transmembrane proteins • Recombinant antibodies • antibody fragments |