Custom Recombinant Protein Expression Services
Why choose Sino Biological for your protein production service?
• Sino has over 10 years' experience resulting in the successful development and production of 6000+ recombinant proteins.
• One-stop service from gene to protein
• Multiple protein expression systems available including bacteria, yeast, baculovirus-insect and mammalian expression systems
• Multiple purification systems (30+) from which to choose
• Comprehensive research and development capabilities with over 1000 new proteins generated per year
• Almost 100 bioreactors with various volume capacities ranging from 2 to 1000 L to meet high-throughput and large-scale production requirements
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| Overview of Recombinant Protein Expression: Key Influencing Factors, Case Studies and FAQs |
| Download Sino Biological's newest Brochure (2019): Strategies to Obtain High-Quality Recombinant Proteins: Protocols and Case Studies |
All protein production services
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- • Gene synthesis and codon optimization (optional)
- • Vector construction
- • Expression evaluation
- • Protein expression and purification
- • Large-scale expression and purification (optional)
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- • Gene synthesis and codon optimization (optional)
- • Vector construction
- • Expression and purification
- • Large-scale expression and purification (optional)
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- • Gene synthesis and codon optimization (optional)
- • Vector construction
- • Virus packaging and amplification
- • Expression and purification
- • Large-scale expression and purification (optional)
Case Studies of Recombinant Protein Production Service Case Studies
Case study of E.coli protein A expression More details>>>
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SEC-HPLC >98.5% 
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Cell bioactivity: EC50=17pg/mL 
Case study of Baculovirus-Insect protein A expression More details>>>
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SEC-HPLC >93% 
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Cell bioactivity: EC50=7ng/mL 
Case study of HEK293 transient protein A expression More details>>>
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SEC-HPLC >98.2% 
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SDS-PAGE >98.7% 
Custom Recombinant Protein Production Service Advantages
| E.coli Protein Expression Service | • Over 10 years' experience of protein expression |
| • Multiple protein purification and refolding technologies | |
| • High-throughput and large-scale protein expression and production | |
| • One-stop service from gene to purified protein | |
| • Multiple expression vectors and E.coli cells to meet various expression needs |
| Mammalian Transient Protein Expression Service | • High-efficiency expression vectors |
| • Optimized mammalian expression systems | |
| • High-throughput and large-scale production | |
| • High yield expression with post-translational modification and correct folding | |
| • One-stop service from gene sequence to purified protein |
| Baculovirus-Insect Cell Protein Expression Service | • High-efficiency expression of intracellular and extracellular proteins |
| • Multiple insect cells: Sf9，Sf21，Hi-5 | |
| • High-throughput and large-scale protein production | |
| • Optimized expression vectors and higher-titre virus packaging |
Recombinant Protein Production Systems Reference
| Expression Systems | E.coli | Yeast | Baculovirus-insect | Mammalian |
| Prokaryotic | Eukaryotic | Eukaryotic | Eukaryotic | |
| Advantages | • Low cost • Rapid expression • Easy scale-up • Most widely used system for recombinant protein production |
• Low cost • Rapid expression • High yield • Diverse post-translational modifications |
• High capacity genes • Soluble proteins • Suitable for toxic proteins • Post-translational modifications similar to mammalian systems |
• Soluble protein • Lower endotoxin • Better bioactivity • Comprehensive post-translational modifications • Transient & stable expression |
| Challenges | • Inclusion bodies • Lack of eukaryotic post-translational modifications • Difficult to express higher MW proteins |
• Improper glycosylation • High mannose modification |
• More demanding culture conditions • Lack of partial glycosylation |
• More demanding culture conditions |
| Most Common Applications | • Bacterial proteins • Antigen proteins • Cytokines • Enzymes |
• Cytokines • Lower MW proteins • Enzymes |
• Cytoplasmic proteins • Toxic proteins • Transmembrane proteins • Secretory proteins • Kinases |
• Secretory proteins • Extracellular domain of transmembrane proteins • Recombinant antibodies • antibody fragments |
