Yeast Protein Expression Service (NOT available temporarily)
Yeast Protein Expression Service Introduction
Yeast protein expression system, combining with the advantages of both prokaryotic system and eukaryotic system, is an economical and high-efficiency eukaryotic protein expression system. It possesses features of low-cost media, low demands for culturing conditions and suitabilty to scale up. Compared with prokaryotic system, yeast protein expression system is more capable of partial post-translational modification, such as glycosylation, acylation, lipidation, and phosphorylation. It is useful for expression of high-valued protein that closely resembles natural structure.
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Yeast Protein Expression Service Items
| Service procedures | Specification | Timeline | Deliverables |
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| ① Gene synthesis and codon optimization (optional) |
1-2 weeks | • Sequencing report | |
| ② Vector construction |
• Clone cDNA into expression vector • Plasmid sequencing • Large-scale plasmid preparation |
1-2 weeks | |
| ③ Vector linearization and transformation |
• Vector linearization • Transforming linearized vector into suitable strain • Identification of positive transformants via PCR • High-copy transformation |
1 weeks | |
| ④ Expression and purification |
• Pilot scale expression, purification and condition optimization • SDS-PAGE and UV analysis |
1-2 weeks | • Feasibility report • 0.1-0.5 mg sample (If expression is feasible and sample remains from QC testing) |
| ⑤ Large-scale expression and purification (optional) |
2-3 weeks | • Purified protein • SDS-PAGE report • CoA |
Yeast Protein Expression Service Procedures
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Communicate about protein expression requirements |
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Sign contract and make prepayment |
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Carry out pilot study for protein expression |
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Feedback pilot study result and ship sample (if feasible) |
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Bulk production quotation |
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Bulk production based on customer requirements |
Yeast Protein Expression Service Advantages
- Over 10 years' experience of protein expression
- One-stop service from gene to purified protein
- Efficient expression vectors with high yield
- High-throughput and large-scale protein production
Contact Us
Tel
+86-010-50911666
Fax
+86-010-50953280
Email
cro-service@sinobiological.com
