Yeast Protein Expression Service

Yeast Protein Expression Service Introduction

Yeast protein expression system, combining with the advantages of both prokaryotic system and eukaryotic system, is an economical and high-efficiency eukaryotic protein expression system. It possesses features of low-cost media, low demands for culturing conditions and suitabilty to scale up. Compared with prokaryotic system, yeast protein expression system is more capable of partial post-translational modification, such as glycosylation, acylation, lipidation, and phosphorylation. It is useful for expression of high-valued protein that closely resembles natural structure.

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Yeast Protein Expression Service Items

Service procedures	Specification	Timeline	Deliverables
① Gene synthesis and codon optimization (optional)		1-2 weeks	• Sequencing report
② Vector construction	• Clone cDNA into expression vector • Plasmid sequencing • Large-scale plasmid preparation	1-2 weeks
③ Vector linearization and transformation	• Vector linearization • Transforming linearized vector into suitable strain • Identification of positive transformants via PCR • High-copy transformation	1 weeks
④ Expression and purification	• Pilot scale expression, purification and condition optimization • SDS-PAGE and UV analysis	1-2 weeks	• Feasibility report • 0.1-0.5 mg sample (If expression is feasible and sample remains from QC testing)
⑤ Large-scale expression and purification (optional)		2-3 weeks	• Purified protein • SDS-PAGE report • CoA

Yeast Protein Expression Service Advantages

Years of experience of protein expression
One-stop service from gene to purified protein
Efficient expression vectors with high yield
High-throughput and large-scale protein production

Yeast Protein Expression Service Procedures


Communicate about protein expression requirements		Sign contract and make prepayment		Carry out pilot study for protein expression		Feedback pilot study result and ship sample (if feasible)		Bulk production quotation		Bulk production based on customer requirements

	Communicate about protein expression requirements

	Sign contract and make prepayment

	Carry out pilot study for protein expression

	Feedback pilot study result and ship sample (if feasible)

	Bulk production quotation

	Bulk production based on customer requirements