Yeast Protein Expression Service (NOT available temporarily)

Yeast Protein Expression Service Introduction

Yeast protein expression system, combining with the advantages of both prokaryotic system and eukaryotic system, is an economical and high-efficiency eukaryotic protein expression system. It possesses features of low-cost media, low demands for culturing conditions and suitabilty to scale up. Compared with prokaryotic system, yeast protein expression system is more capable of partial post-translational modification, such as glycosylation, acylation, lipidation, and phosphorylation. It is useful for expression of high-valued protein that closely resembles natural structure.

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Yeast Protein Expression Service Items

Service procedures Specification Timeline Deliverables

Gene synthesis and codon optimization (optional)
  1-2 weeks • Sequencing report

Vector construction
• Clone cDNA into expression vector
• Plasmid sequencing
• Large-scale plasmid preparation
1-2 weeks

Vector linearization and transformation
• Vector linearization
• Transforming linearized vector into suitable strain
• Identification of positive transformants via PCR
• High-copy transformation
1 weeks

Expression and purification
• Pilot scale expression, purification and condition optimization
• SDS-PAGE and UV analysis
1-2 weeks • Feasibility report
• 0.1-0.5 mg sample
(If expression is feasible and sample remains from QC testing)

Large-scale expression and purification (optional)
  2-3 weeks • Purified protein
• SDS-PAGE report
• CoA

Yeast Protein Expression Service Advantages

  • Years of experience of protein expression
  • One-stop service from gene to purified protein
  • Efficient expression vectors with high yield
  • High-throughput and large-scale protein production

Yeast Protein Expression Service Procedures

Communicate about protein expression requirements
Sign contract and make prepayment
Carry out pilot study for protein expression
Feedback pilot study result and ship sample (if feasible)
Bulk production quotation
Bulk production based on customer requirements

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