Yeast protein expression system, combining with the advantages of both prokaryotic system and eukaryotic system, is an economical and high-efficiency eukaryotic protein expression system. It possesses features of low-cost media, low demands for culturing conditions and suitabilty to scale up. Compared with prokaryotic system, yeast protein expression system is more capable of partial post-translational modification, such as glycosylation, acylation, lipidation, and phosphorylation. It is useful for expression of high-valued protein that closely resembles natural structure.
Service procedures | Specification | Timeline | Deliverables |
---|---|---|---|
① Gene synthesis and codon optimization (optional) |
1-2 weeks | • Sequencing report | |
② Vector construction |
• Clone cDNA into expression vector • Plasmid sequencing • Large-scale plasmid preparation |
1-2 weeks | |
③ Vector linearization and transformation |
• Vector linearization • Transforming linearized vector into suitable strain • Identification of positive transformants via PCR • High-copy transformation |
1 weeks | |
④ Expression and purification |
• Pilot scale expression, purification and condition optimization • SDS-PAGE and UV analysis |
1-2 weeks | • Feasibility report • 0.1-0.5 mg sample (If expression is feasible and sample remains from QC testing) |
⑤ Large-scale expression and purification (optional) |
2-3 weeks | • Purified protein • SDS-PAGE report • CoA |
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Communicate about protein expression requirements | Sign contract and make prepayment | Carry out pilot study for protein expression | Feedback pilot study result and ship sample (if feasible) | Bulk production quotation | Bulk production based on customer requirements |
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Communicate about protein expression requirements |
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|
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Sign contract and make prepayment |
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|
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Carry out pilot study for protein expression |
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|
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Feedback pilot study result and ship sample (if feasible) |
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|
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Bulk production quotation |
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|
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Bulk production based on customer requirements |
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